MonoRab™ 兔单克隆抗体
兔单克隆抗体（mAbs）凭借其亲和力高、特异性强、及识别抗原表位更丰富等独特优势，在基础科研、诊断和医疗等领域都得到广泛的应用。GenScript是全球第二家利用自主知识产权的杂交瘤细胞技术开发兔单克隆抗体的公司。
我们采用基于杂交瘤细胞技术的MonoRab™技术平台，通过筛选获得动物自身亲和力成熟的特异性抗体，开发出性能优越的兔单克隆抗体。该平台在以小分子药、生物药、甚至小鼠体内的非免疫性抗原等多种靶点的抗体开发中，具有非常巨大的技术优势。
MonoRab™ Platform VS. Mouse Monoclonal Technology
| Comparison | MonoRab™ platform | Mouse mAb technology |
|---|---|---|
| Antigen Recognization | Capable of recognizing a variety of difficult antigens:
|
Limited immune response |
| Antibody Sensitivity | High It enables the detection of less abundant antigens with a minimum amount of antibody, making the MonoRab™ antibody a cost-effective reagent. |
Medium-High |
| Antibody Specificity | High This reduces background signals and allow for the precise targeting of desired antigens |
Medium-High |
| Antibody Affinity | Picomolar KD values This allows for the preservation of antibody-antigen interaction for a robust detection and function |
Nano or micromolar KD values |
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- Lane 1: MonoRab™ Anti-His antibody (4 μg) + His tagged fusion protein
- Lane 2: Rabbit IgG (4 μg) + His tagged fusion protein
- Lane 3: His tagged fusion protein (Input)
Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRab™ Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit ( A01857-40)
Comparison of IHC using beta actin rabbit mAb on monkey heart tissue, 0.25 µg/ml, and hematoxylin counterstain.
GenScript MonoRab™ Beta Actin Antibody, mAb, Rabbit ( A01865-40) shows stronger signal with low background compared with other suppliers.
MonoRab™ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit ( A01895-40) binds with Bevacizumab. While the antibody does not recognize the human IgG Fc fragment (data not shown).
EC50= 7.280 ng/ml
MonoRab™ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit ( A01896-40) blocking with Vascular Endothelial Growth Factor 165 (VEGF 165).
EC50= 46.16 ng/ml
Standard curve of Bevacizumab Sandwich ELISA. It is developed by using MonoRab™ Anti-Bevacizumab Antibody (30E1), mAb, Rabbit ( A01895-40) and MonoRab™ Anti-Bevacizumab Antibody (46E3) [Biotin], mAb, Rabbit ( A01896-40) as the capture and detection antibodies, respectively.
The sensitivity of detecting Bevacizumab is up to 20 pg/ml.
